Serpent.AI is a simple yet powerful, novel framework to assist developers in the creation of game agents. Turn ANY video game you own into a sandbox environment ripe for experimentation, all with familiar Python code. The framework's raison d'être is first and foremost to provide a valuable tool for Machine Learning & AI research. It also turns out to be ridiculously fun to use as a hobbyist (and dangerously addictive; a fair warning)! The framework features a large assortment of supporting modules that provide solutions to commonly encountered scenarios when using video games as environments as well as CLI tools to accelerate development. It provides some useful conventions but is absolutely NOT opiniated about what you put in your agents: Want to use the latest, cutting-edge deep reinforcement learning algorithm? ALLOWED. Want to use computer vision techniques, image processing and trigonometry? ALLOWED. Want to randomly press the Left or Right buttons? sigh ALLOWED. To top it all off, Serpent.AI was designed to be entirely plugin-based (for both game support and game agents) so your experiments are actually portable and distributable to your peers and random strangers on the Internet.